全文获取类型
收费全文 | 85820篇 |
免费 | 8771篇 |
国内免费 | 22865篇 |
学科分类
生物科学 | 117456篇 |
出版年
2024年 | 173篇 |
2023年 | 1715篇 |
2022年 | 2624篇 |
2021年 | 4793篇 |
2020年 | 3824篇 |
2019年 | 4508篇 |
2018年 | 3688篇 |
2017年 | 3127篇 |
2016年 | 3884篇 |
2015年 | 5421篇 |
2014年 | 7064篇 |
2013年 | 6629篇 |
2012年 | 8512篇 |
2011年 | 8004篇 |
2010年 | 5562篇 |
2009年 | 5433篇 |
2008年 | 5947篇 |
2007年 | 5513篇 |
2006年 | 4957篇 |
2005年 | 4187篇 |
2004年 | 3270篇 |
2003年 | 2934篇 |
2002年 | 2428篇 |
2001年 | 2217篇 |
2000年 | 2088篇 |
1999年 | 1551篇 |
1998年 | 834篇 |
1997年 | 693篇 |
1996年 | 615篇 |
1995年 | 595篇 |
1994年 | 534篇 |
1993年 | 430篇 |
1992年 | 551篇 |
1991年 | 412篇 |
1990年 | 350篇 |
1989年 | 317篇 |
1988年 | 267篇 |
1987年 | 246篇 |
1986年 | 230篇 |
1985年 | 226篇 |
1984年 | 170篇 |
1983年 | 154篇 |
1982年 | 152篇 |
1981年 | 66篇 |
1980年 | 55篇 |
1979年 | 63篇 |
1976年 | 46篇 |
1974年 | 54篇 |
1973年 | 45篇 |
1972年 | 53篇 |
排序方式: 共有10000条查询结果,搜索用时 501 毫秒
81.
在干旱、半干旱荒漠生态系统中,灌丛作为一种重要的植被类型,其独特的形态和生理适应特性能够有效促进退化生态系统结构与功能的恢复。土壤动物是荒漠生态系统中不可或缺的重要组成部分,对促进灌丛"肥岛"演变具有重要的生态作用,有利于灌丛生态功能的发挥及退化生态系统的恢复。近年来,国内外学者对荒漠灌丛微生境土壤动物的研究逐步深入,取得大量的研究成果。在此基础上,首先综述荒漠灌丛微生境土壤动物群落分布和生态功能,总结灌丛与土壤动物分布间作用关系的数学模型,针对荒漠灌丛土壤动物研究中存在的问题提出了未来可能的研究方向和建议。 相似文献
82.
83.
84.
Mengnan Wang Dongjie Li Mingyue Zhang Wenzhi Yang Yali Cui Shijie Li 《Animal genetics》2015,46(4):354-360
The CDKN1C gene encodes a cyclin‐dependent kinase inhibitor and is one of the key genes involved in the development of Beckwith–Wiedemann syndrome and cancer. In this study, using a direct sequencing approach based on a single nucleotide polymorphism (SNP) at genomic DNA and cDNA levels, we show that CDKN1C exhibits monoallelic expression in all seven studied organs (heart, liver, spleen, lung, kidney, muscle and subcutaneous fat) in cattle. To investigate how methylation regulates imprinting of CDKN1C in cattle, allele‐specific methylation patterns in two putative differential methylation regions (DMRs), the CDKN1C DMR and KvDMR1, were analyzed in three tissues (liver, spleen and lung) using bisulfite sequencing PCR. Our results show that in the CDKN1C DMR both parental alleles were unmethylated in all three analyzed tissues. In contrast, KvDMR1 was differentially methylated between the two parental alleles in the same tissues. Statistical analysis showed that there is a significant difference in the methylation level between the two parental alleles (P < 0.01), confirming that this region is the DMR of KvDMR1 and that it may be correlated with CDKN1C imprinting. 相似文献
85.
86.
Yihao Yang Ziyan Shen Youguang Li Chenda Xu Han Xia Hao Zhuang Shengyuan Sun Min Guo Changjie Yan 《植物学报(英文版)》2022,64(10):1860-1865
Rice eating and cooking quality (ECQ) is a major concern of breeders and consumers, determining market competitiveness worldwide. Rice grain protein content (GPC) is negatively related to ECQ, making it possible to improve ECQ by manipulating GPC. However, GPC is genetically complex and sensitive to environmental conditions; therefore, little progress has been made in traditional breeding for ECQ. Here, we report that CRISPR/Cas9-mediated knockout of genes encoding the grain storage protein glutelin rapidly produced lines with downregulated GPC and improved ECQ. Our finding provides a new strategy for improving rice ECQ. 相似文献
87.
88.
Yoko Yamauchi Hikari Kimoto Xianyu Yang Sergey Filkin Yuri Utkin Tai Kubo 《Bioscience, biotechnology, and biochemistry》2016,80(1):158-161
Three-finger toxins (3FTxs) are one of the major components in snake venoms. In this study, we isolated a cDNA encoding a short-chain 3FTx, Pr-SNTX, from Pseudechis rossignolii. The amino acid sequence of Pr-SNTX is nearly identical to that of its ortholog in Pseudechis australis. Pr-SNTX protein inhibited muscle-type (α2βδε), but not neuronal α7 nicotinic acetylcholine receptor (nAChR) activity. 相似文献
89.
90.
Xiuling Li Songbai Yang Zhonglin Tang Kui Li Max F. Rothschild Bang Liu Bin Fan 《Animal genetics》2014,45(3):329-339
Due to the direction, intensity, duration and consistency of genetic selection, especially recent artificial selection, the production performance of domestic pigs has been greatly changed. Therefore, we reasoned that there must be footprints or selection signatures that had been left during domestication. In this study, with porcine 60K BeadChip genotyping data from both commercial Large White and local Chinese Tongcheng pigs, we calculated the extended haplotype homozygosity values of the two breeds using the long‐range haplotype method to detect selection signatures. We found 34 candidate regions, including 61 known genes, from Large White pigs and 25 regions comprising 57 known genes from Tongcheng pigs. Many selection signatures were found on SSC1, SSC4, SSC7 and SSC14 regions in both populations. According to quantitative trait loci and network pathway analyses, most of the regions and genes were linked to growth, reproduction and immune responses. In addition, the average genetic differentiation coefficient FST was 0.254, which means that there had already been a significant differentiation between the breeds. The findings from this study can contribute to further research on molecular mechanisms of pig evolution and domestication and also provide valuable references for improvement of their breeding and cultivation. 相似文献